SSR and SRAP marker-based linkage map of Vitis vinifera L.
نویسندگان
چکیده
An F1 population was created by the cross '87-1' × '9-22'. The female parent '87-1' was an extremely early maturing cultivar with strong flavour. The male parent was an excellent breeding line producing large berries maturing late. The mapping population included 149 randomly chosen individuals. Molecular genetic map for each parent and the consensus map were constructed using simple sequence repeat and sequence-related amplified polymorphism markers by software JoinMap 3.0. The '87-1' map covers a total length of 1272.9 cM distributed in 21 linkage groups and consists of 163 molecular markers with an average distance between adjacent markers of 8.9 cM. The '9-22' map covers a total length of 1267.4 cM distributed in 20 linkage groups and consists of 158 molecular markers with an average distance between adjacent markers of 9.1 cM. The consensus map covers a total length of 1537.1 cM distributed in 21 linkage groups and one doublet and consists of 217 molecular markers with an average distance of 7.8 cM between adjacent markers. The length of the linkage groups is 69.8 cM on average. The map covers the 19 chromosomes of the Vitis genome and can lay a solid foundation for further studies such as quantative trait loci (QTL) mapping of correlated traits and marker-assisted selection.
منابع مشابه
Ssr and Srap Marker Based Linkage Map of Vitis Amurensis Rupr
SSR (Simple Sequence Repeat) markers and SRAP (Sequence Related Amplified Polymorphism) markers were used to construct a genetic linkage map of Vitis amurensis Rupr. The mapping population consists of 94 individuals derived from the selfing of Beibinghong, a V. amurensis cultivar. The map covers a total length of 1123cM on 21 linkage groups with an average distance between adjacent markers of 4...
متن کاملA dense SNP-based genetic linkage map of grapevine (Vitis vinifera L.) anchoring Pinot Noir BAC contigs
The construction of a dense genetic map for Vitis vinifera and its anchoring to a BAC-based physical map is described: it includes 994 loci mapped onto 19 linkage groups, corresponding to the basic chromosome number of Vitis. Spanning 1,245 cM with an average distance of 1.3 cM between adjacent markers, the map was generated from the segregation of 483 SNP-based genetic markers, 132 SSRs, and 3...
متن کاملGenetic linkage map of EST-SSR and SRAP markers in the endangered Chinese endemic herb Dendrobium (Orchidaceae).
Dendrobium officinale is an endangered orchid from southeast Asia that is known for its medicinal properties in traditional Chinese medicine. We constructed an integrated genetic linkage map of an F(1) population derived from an interspecific cross between D. officinale and D. aduncum (both, 2n = 38), using expressed sequence tag-simple sequence repeats (EST-SSR) and sequence-related amplified ...
متن کاملDetection of Polymorphism in Ancient Tempranillo Clones (Vitis vinifera L.) Using Microsatellite and Retrotransposon Markers
Tempranillo is one of the most widely cultivated grapevine varieties in Spain. After several years of clone selection, some highly recommended old clones have been identified in terms of both their qualitative and production characteristics. This study was designed to discriminate among 28 ancient clones of the cultivar Tempranillo (Vitis vinifera). DNA samples from clones were analysed using t...
متن کاملIdentification of QTLs for grain yield and some agro-morphological traits in sunflower (Helianthus annuus L.) using SSR and SNP markers
Many agriculturally important traits are complex, affected by many genes and the environment. Quantitative trait loci (QTL) mapping is a key tool for studying the genetic structure of complex traits in plants. In the present study QTLs associated with yield and agronomical traits such as leaf number, leaf length, leaf width, plant height, stem and head diameter were identified by using 70 recom...
متن کامل